Bioluminescence Assays and Reagents
Cayman Chemical LucIfer Yelow CH 25mg
A fluorescent probe; contains a free hydrazido group that reacts with aliphatic aldehydes at room temperature; ex/em = 430/540 nm, respectively; been used as a biological tracer to monitor the branching pattern and regeneration of neurons, gap junction detection and characterization, and selective ablation of cells following aldehyde fixation
Indigo Biosciences Human Tropomyosin Receptor Kinase C (TrkC) Reporter Assay System, All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Tropomyosin Receptor Kinase C reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against TrkC. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
GeneCopoeia PURIF LENTIVIRAL PRTCLS 25ULX4
HLUC-Lv105 Firefly Luciferase Lentifect™ Purified Lentiviral Particles (25 µl x 4 vials; CMV promoter, Puro marker
Cayman Chemical Kaempferol 3-O-galactosIde 5mg
A flavonoid with diverse biological activities; inhibits sPLA2 (IC50 = 17.6 µM); decreases protein levels of Akt and induces apoptosis in NCI H460 NSCLC cells at 12.5, 25, and 50 µM; scavenges DPPH radicals and superoxide anions in cell-free assays at 200 UG/ml; reduces TPA-induced EAr edema and carrageenan-induced paw edema in mice at 150 mg/kg
Indigo Biosciences Human LXR Reporter Assays Panel All-inclusive Cell-Based Luciferase Reporter Assay Kit, Including LXRa & LXRb, each in 1x48 96-well format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Liver X Receptor Panel (LXR alpha, LXR beta) assay kit is an all-inclusive firefly luciferase reporter assay system that includes in addition to reporter cells for each LXR, optimized growth media, media for diluting test compounds, a control agonist for each LXR, luciferase detection reagent, a cell culture-ready assay plate in strip-well format so that (if preferred) LXR alpha and LXR beta assays may be performed at different times, detailed protocol, and a protocol quick guide. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Apexbio Technology LLC Firefly Luciferase mRNA (ARCA, 5mCTP, UTP), 100ug (1mg/mL)
Firefly Luciferase mRNA (ARCA, 5mCTP, UTP) expresses luciferase protein which is initially extracted from firefly Photinus pyralis. The enzyme catalyzes ATP-dependent D-luciferin oxidation to yield oxyluciferin, a singlet-excited compound that emits light when returning to its ground state. Firefly Luciferase is a frequently used bioluminescent reporter for gene regulation and function study. It is applicable in assays for gene expression, cell viability and in vivo imaging etc. Our Firefly Luciferase mRNA is provided at a concentration of 1mg/ml, it is ARCA capped, polyA-tailed and incorporated with modified nucleotides. The capping of ARCA (anti-reverse cap analog) ensures high translation efficacy, while the addition of 5mCTP/UTP and poly(A) tail reduces host cell immune response and enhance mRNA stability. Other sizes are available. Please inqury us for quote.
Amsbio LLC CMV-Luciferase (firefly)-2A-GFP (Bsd)/ Concentrated Lentivirus
CMV-Luciferase (firefly)-2A-GFP (Bsd) in vivo ready
Cayman Chemical QuercetIn 3-D-galactosIde 25mg
A natural 3-O-galactoside of quercetin that has powerful antioxidant action through its ability to scavenge free radicals (IC50s = 3.54 and 5.44 UG/ml in ABTS and DPPH assays, respectively)